Precise conjugation, made simple.
Site-specific enzymatic bioconjugation for programmable payload attachment.
Applications
Enzymatic precision and efficiency for your bioconjugation experiments.
Most site-specific methods achieve precision only at the cost of long tags, costly custom substrates, or fussy chemistry. Enzura uses a proprietary bacterial enzyme that recognises a short peptide motif and covalently installs an alkyne or azide handle on a single threonine within it. The handle-bearing substrate is itself made enzymatically, from NAD⁺ and a simple alcohol, in the same tube.
Site-specific by design
Programmable tag
Works at the N-terminus, the C-terminus or in an internal loop.
One-pot reaction with low-cost substrates
No custom synthesis. The click-ready substrate is made enzymatically from bulk NAD⁺ (~$1/g) and a commodity alkyne or azide alcohol.
Stable bond, minimal scar
A phosphodiester linkage that survives phosphatase and nuclease treatment and a broad range of pH, temperature and buffer additives.
Three steps, one pot.
Steps 1 and 2 run together in a single tube with no intermediate purification. Step 3 is standard click chemistry.
Make the click substrate
We convert NAD⁺ and a small alkyne or azide alcohol into a click-ready dinucleotide. Entirely enzymatic: no organic synthesis, no protecting groups.
Install the handle
Our proprietary enzyme recognises a small proprietary tag and transfers the handle-bearing phosphoribose onto its threonine. Unreacted NAD⁺ is not a substrate, so the two enzymes coexist without cross-talk.
Conjugate your payload
React the alkyne or azide with any complementary partner: dyes, biotin, oligonucleotides, another tagged protein, or a functionalised resin.
Make precise conjugation simple, cheap, and accessible.
Precise bioconjugation shouldn’t depend on long protein tags, custom substrates, or specialized chemistry. Enzura Biotech aims to change that.
Let's build the next bioconjugate, together.
We're looking for partners, collaborators, and investors. Tell us your target and payload and we'll tell you whether the tag fits.
Or email us directly at info@enzurabiotech.com